Genotyping
The CGRB Core Labs use two ABI 3100 capillary DNA sequencers to resolve and analyze fluorescently labeled DNA fragments for genotyping applications.
ABI Genescan® software is used to size fragments based on internal lane standard. Further analysis with Genotyper® software can be used for microsatellite analysis, AFLP analysis, gene expression profiling, mutation detection, and mutation screening. Genotyper® can set up categories, sort peak data, statistically analyze data, filter out extra peaks generated with microsatellites, and set up tables with correlated peak and marker information that can be exported for further analysis with other software.
We are currently running microsatellites, AFLPs and SSCPs on a diverse set of organisms, including salmon, oysters, poplar, sunflower, birds, snakes, potatoes, barley, wheat, hazelnuts, fungi and soil and marine microorganisms.
New users of the Genotyping Service should consult with Caprice Rosato. Users should be aware of plate format, fluorescent primer, and fragment quality requirements for analysis using the robotic ABI 3100 machines.
All ordering is web-based. You must contact Matthew Peterson in order to get a login and password. Then use the Core Labs web site to place your order and to check the status of your order.