Sample Quality
Contact person for this service is Caprice Rosato.
- Contaminants such as high salt, poor DNA or PCR stutter can reduce data quality.
- Samples should be properly diluted before submitting
- TRFLPs and similar samples should be column purified before submitting
- Column cleaning can be done by ordering through the CGRB Core Labs Services site
- Dilutions
- It is important that you empirically determine what DNA concentrations are optimal for your project
- When starting a new project, we suggest doing dilutions series test (i.e. four samples, each diluted 1:5, 1:25, 1:125, 1:625)
- Consequences: if your samples are too concentrated, it may fail to analyze and/or the data will not be precise. High signal in one color can lead to spectral overlap into other colors, possibly leading to confusing results.
- Optimal peak height is between 300-3000 RFUs