Sample Orientation

Orientation is very important in the capillary system and is dependent on using either 96 or 384-well plate

• In a 96-well plate, a single run is loaded from two adjacent columns. Each run must begin with an odd-numbered column (i.e. 1+2, 3+4, etc.) for a maximum of 6 runs per plate.
  • You need not start at well A1 every time: if you had 32 samples they would be 9A-12H, as long as the data sheet corresponded.

 

• In a 384-well plate, the orientation is more complated, especially if you are running less than a full plate. Because the well numbers are doubled, both horizontally and vertically within the same overall plate dimension, and because the capillaries are unable to move, the samples for each run are not in adjacent wells but rather in wells separated by a well. For example, using A01 for the first sample the rest of the samples for that run are: A03, C01, C03, E01, E03, G01, G03, I01, I03, K01, K03, M01, M03, O01, O03. The next run could being in B01 or in A02 and follow the same pattern skipping a well in between each sample for that run. The runs and analysis should go smoothly as long as your data sheet corresponds properly to your plate full of samples.