High Throughput Sequencing (HTS)
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The CGRB recently acquired an Illumina 1G Genome Analyzer. The system is operational and ready for a variety of applications. The sequencing process takes place on a flow cell with 8 channels (one channel must be used for control DNA), each of which may contain a different sample. Over 260 Mb of raw sequence data are generated per channel, although usable, high-quality data will be less. For expression profiling, up to 7.5 million tags are sequenced per channel. |
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Illumina sells four different sample prep kits: Genomic DNA, NlaIII Gene Expression, DpnII Gene Expression, and Small RNA. Reagents can be purchased directly from Illumina, or can be coordinated within the CGRB. Please contact Mark Dasenko for more information and assistance with getting started. Additionally, please be well aware of the quantity of image and sequence data that will be generated during a run (nearly 1 Tb). This will require significant computational power and expertise. The CGRB will provide a number of service, software and hardware tools to facilitate capture and analysis of data. However, each group should consider their long-term computational programming and utilization needs, and plan accordingly with personnel. To help the Illumina 1G user community, we established an HTS Group that meets bi-weekly and shares information. Please contact Scott Givan for more information and recommendations, or to join the HTS group.
An overview of the 1G workflow and approximate durations of steps for a typical |
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