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Nimblegen array processing

The Nimblegen Hybridization Station (4-bay) in the CGRB Core Lab can process high-density arrays such as those available from Nimblegen.  Contact Caprice Rosato for information about using this service. 

                                             

 Processing Nimblegen Microarrays

The 4-bay Nimblegen hybridization station is designed to provide optimal hybridization on high-density arrays fabricated by Nimblegen.  The Nimblegen hybridization station uses a mixer for uniform distribution of sample across the array.  Arrays (commercially available or spotted in-house) that conform to the dimensions of Nimblegen mixers and slide chemistry can be hybridized using this instrument.   Labeling, hybridization, scanning and data extraction procedures can be provided as a full service or as a partial service according to the needs of the researcher.

Nimblegen arrays can be by quote directly from Nimblegen and they can be shipped directly to the CGRB core lab.  Custom arrays can be designed with a five (5) chip minimum purchase required.

 

There are currently four (4) configurations for Nimblegen arrays, corresponding to the necessary mixer (see information on mixers here):

  • 1-plex array consisting of one array per chip with 385K features
  • 4-plex array consisting of four identical arrays per chip with 72K features each array
  • 12-plex array consisting of 12 identical arrays per chip with 135K features each array
  • HD2 array consisting of one array per chip with 2.1 million features

 

Nimblegen arrays offer the following applications:

  • Gene Expression
  • Comparative Genome Hybridization (CGH) including Tiling Arrays
  • DNA Methylation (Epigenetics) including Tiling Arrays
  • Chromatin ImmunoPrecipitation (ChIP) including Tiling Arrays

 

Sequence Capture using Nimblegen arrays is available through NimbleGen.