Nimblegen array processing
The Nimblegen Hybridization Station (4-bay) in the CGRB Core Lab can process high-density arrays such as those available from Nimblegen. Contact Caprice Rosato for information about using this service.

Processing Nimblegen Microarrays
The 4-bay Nimblegen hybridization station is designed to provide optimal hybridization on high-density arrays fabricated by Nimblegen. The Nimblegen hybridization station uses a mixer for uniform distribution of sample across the array, and any array (commercially available or spotted in-house) that conforms to the dimensions of one of the Nimblegen mixers can be hybridized using this instrument. Labeling, hybridization, scanning and data extraction procedures can be provided as a full service or as a partial service according to the needs of the researcher.
Nimblegen arrays can be ordered through the CGRB or directly from Nimblegen. Custom arrays can be designed with a five (5) chip minimum purchase required.
There are currently four (4) configurations for Nimblegen arrays, corresponding to the necessary mixer (see information on mixers here):
- 1-plex array consisting of one array per chip with 385K features
- 4-plex array consisting of four identical arrays per chip with 72K features each array
- 12-plex array consisting of 12 identical arrays per chip with 135K features each array
- HD2 array consisting of one array per chip with 2.1 million features
Nimblegen arrays offer the following applications:
- Gene Expression
- Comparative Genome Hybridization (CGH) including Tiling Arrays
- DNA Methylation (Epigenetics) including Tiling Arrays
- Chromatin ImmunoPrecipitation (ChIP) including Tiling Arrays
Soon Sequence Capture using Nimblegen arrays will also be available.

