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Nimblegen array processing

The Nimblegen Hybridization Station (4-bay) in the CGRB Core Lab can process high-density arrays such as those available from Nimblegen.  Contact Caprice Rosato for information about using this service. 

                                             

 Processing Nimblegen Microarrays

The 4-bay Nimblegen hybridization station is designed to provide optimal hybridization on high-density arrays fabricated by Nimblegen.  The Nimblegen hybridization station uses a mixer for uniform distribution of sample across the array, and any array (commercially available or spotted in-house) that conforms to the dimensions of one of the Nimblegen mixers can be hybridized using this instrument.  Labeling, hybridization, scanning and data extraction procedures can be provided as a full service or as a partial service according to the needs of the researcher.

Nimblegen arrays can be ordered through the CGRB or directly from Nimblegen.  Custom arrays can be designed with a five (5) chip minimum purchase required.

 

There are currently four (4) configurations for Nimblegen arrays, corresponding to the necessary mixer (see information on mixers here):

  • 1-plex array consisting of one array per chip with 385K features
  • 4-plex array consisting of four identical arrays per chip with 72K features each array
  • 12-plex array consisting of 12 identical arrays per chip with 135K features each array
  • HD2 array consisting of one array per chip with 2.1 million features

 

Nimblegen arrays offer the following applications:

  • Gene Expression
  • Comparative Genome Hybridization (CGH) including Tiling Arrays
  • DNA Methylation (Epigenetics) including Tiling Arrays
  • Chromatin ImmunoPrecipitation (ChIP) including Tiling Arrays

 

Soon Sequence Capture using Nimblegen arrays will also be available.