Primer Concentration
Concentration of Template and Primer
Supply the Core Labs with a mixture that contains 12 picomoles
of primer, and templates at the concentration listed below, in a
final volume of 12 µL in water. If using one of the
ten supplied primers, provide listed amount of template in 6
µL water. This is double the amount of template and
primer that we actually need so that we can rerun samples if there
are any problems.
We may reject samples if supplied otherwise.
DO NOT ASSUME WE WILL DILUTE YOUR SAMPLES TO THE CORRECT CONCENTRATION. We will run whatever you supply and assume you have diluted correctly.
Concentration of double-stranded template (in water) to supply Core Labs:
| PCR Product | Vector + Insert | ||
| Length | Total DNA in Final Volume |
Length | Total DNA in Final Volume |
| 500 bp | 25 ng | 3 kb - 5 kb | 400 ng |
| 800 bp | 50 ng | 6 kb - 9 kb | 600 ng |
| 1000 bp | 125 ng | 10 kb - 15 kb | 1 µg |
| 2000 bp | 250 ng | >15 kb | 1.2 µg |
| 3000 bp | 350 ng | ||
Please consult with Core Labs staff about amounts of template and primer to use for BAC’s, YAC’s, PAC’s, cosmids, bacterial genomic DNA or M13 preps.
For degenerate primers, add 12 picomoles primer per degeneracy, but do not exceed 100 picomoles.